LOCUS       pLV-[hsa-miR-1302-7]        9126 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7047..7907
                     /label=Amp(R)
     promoter        8108..8648
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5652..5886
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6461..6916)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5958..6088
                     /label=SV40 pA
     misc_feature    6089..6302
                     /label=SV40 ori
     misc_feature    4973..5569
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3591
                     /label=intron
     CDS             3638..4963
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3381
                     /label=hsa-mir-1302-7 precursor
BASE COUNT     2180 a      2365 c      2453 g      2128 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcct cttcccccaa 
     3121 atagggcccc atatttagta actaaaagat attgagcacc agctaaacgt aggtacattt 
     3181 caattcatcc aaacttgaat tccatataaa caacatgttt ttaggacatg tatgtctggt 
     3241 gcaataattg ggacatactt atgctaaaaa aattagtgtt cactatttag ctgagattaa 
     3301 aatttagctg ggcctcctgt tttcatgtgg aaaccctacc ccgaaggcct ctttcctcac 
     3361 catctgtgga atcctggaac ggctagctcg agcttttgga gtacgtcgtc tttaggttgg 
     3421 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg 
     3481 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg 
     3541 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga 
     3601 aaactacccc tctagagtcg agctaccggt cgccaccatg gtgagcaagg gcgaggagga 
     3661 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa 
     3721 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac 
     3781 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc 
     3841 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt 
     3901 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg 
     3961 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa 
     4021 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg 
     4081 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca 
     4141 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc 
     4201 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc 
     4261 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac 
     4321 cggcggcatg gacgagctgt acaaggaccc accggtcgcc accatgaccg agtacaagcc 
     4381 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc 
     4441 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt 
     4501 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 
     4561 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 
     4621 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 
     4681 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 
     4741 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 
     4801 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 
     4861 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg agtgcccgaa 
     4921 ggaccgcgcg acctggtgca tgacccgcaa gcccggtgcc tgagcggccg cgtcgacaat 
     4981 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 
     5041 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 
     5101 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 
     5161 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 
     5221 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 
     5281 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 
     5341 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 
     5401 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 
     5461 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 
     5521 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct 
     5581 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag 
     5641 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt 
     5701 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga 
     5761 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc 
     5821 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct 
     5881 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa 
     5941 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata 
     6001 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 
     6061 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat 
     6121 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 
     6181 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 
     6241 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc 
     6301 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 
     6361 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 
     6421 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca 
     6481 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 
     6541 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 
     6601 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 
     6661 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 
     6721 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 
     6781 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 
     6841 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata 
     6901 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 
     6961 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 
     7021 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 
     7081 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 
     7141 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 
     7201 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 
     7261 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 
     7321 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 
     7381 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 
     7441 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 
     7501 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 
     7561 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 
     7621 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 
     7681 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 
     7741 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 
     7801 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 
     7861 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 
     7921 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 
     7981 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 
     8041 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 
     8101 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 
     8161 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 
     8221 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 
     8281 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 
     8341 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 
     8401 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 
     8461 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 
     8521 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 
     8581 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 
     8641 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 
     8701 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 
     8761 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 
     8821 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc 
     8881 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 
     8941 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta 
     9001 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca 
     9061 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg 
     9121 agctgc 
//
